Fig. 5
From: TFPI2 suppresses breast cancer progression through inhibiting TWIST-integrin α5 pathway
Inhibition ability of TFPI2 on breast cancer progression was reversed by TWIST1 over-expression. a The mRNA expression of TWIST1 was decreased in cells transfected with pcDNA 3.1-TFPI2, while reversed in cells cotransfected with pcDNA 3.1-TFPI2 and pcDNA 3.1-TWIST1. ** represents pcDNA 3.1-TFPI2 vs. pcDNA 3.1-NC or pcDNA 3.1-TFPI2 + pcDNA 3.1-TWIST1 vs. pcDNA 3.1-TFPI2 + pcDNA 3.1-NC, p < 0.01. b pcDNA 3.1-TFPI2 inhibited cell viability of MDA-MB-453 cells, while cotransfection with pcDNA 3.1-TFPI2 and pcDNA 3.1-TWIST1 promoted the cell viability. ** represents pcDNA 3.1-TFPI2 vs. pcDNA 3.1-NC or pcDNA 3.1-TFPI2 + pcDNA 3.1-TWIST1 vs. pcDNA 3.1-TFPI2 + pcDNA 3.1-NC, p < 0.01. c Transfection with pcDNA 3.1-TFPI2 inhibited cell proliferation of MDA-MB-453 cells, while cotransfection with pcDNA 3.1-TFPI2 and pcDNA 3.1-TWIST1 promoted the cell proliferation. ** represents pcDNA 3.1-TFPI2 vs. pcDNA 3.1-NC or pcDNA 3.1-TFPI2 + pcDNA 3.1-TWIST1 vs. pcDNA 3.1-TFPI2 + pcDNA 3.1-NC, p < 0.01. d Transfection with pcDNA 3.1-TFPI2 inhibited cell migration of MDA-MB-453 cells, while cotransfection with pcDNA 3.1-TFPI2 and pcDNA 3.1-TWIST1 promoted the cell migration. ** represents pcDNA 3.1-TFPI2 vs. pcDNA 3.1-NC or pcDNA 3.1-TFPI2 + pcDNA 3.1-TWIST1 vs. pcDNA 3.1-TFPI2 + pcDNA 3.1-NC, p < 0.01. e Transfection with pcDNA 3.1-TFPI2 inhibited cell invasion of MDA-MB-453 cells, while cotransfection with pcDNA 3.1-TFPI2 and pcDNA 3.1-TWIST1 promoted the cell invasion.. ** represents pcDNA 3.1-TFPI2 vs. pcDNA 3.1-NC or pcDNA 3.1-TFPI2 + pcDNA 3.1-TWIST1 vs. pcDNA 3.1-TFPI2 + pcDNA 3.1-NC, p < 0.01. f Protein expression of TWIST1 and integrin α5 were down-regulated in cells transfected with pcDNA 3.1-TFPI2, while up-regulated in cells cotransfected with pcDNA 3.1-TFPI2 and pcDNA 3.1-TWIST1. g Cotransfection with pcDNA 3.1-TFPI2 and shTWIST1 had no significant effect on cell viability of MCF7 cells compared to cells cotransfected pcDNA 3.1-NC with shTWIST1. h Cotransfection with pcDNA 3.1-TFPI2 and shTWIST1 had no significant effect on protein expression of TWIST1 and integrin α5 in MCF7 cells compared to cells cotransfected pcDNA 3.1-NC with shTWIST1. i Cotransfection with pcDNA 3.1-TFPI2 and shTWIST1 had no significant effect on cell proliferation of MCF7 cells compared to cells cotransfected pcDNA 3.1-NC with shTWIST1. j Cotransfection with pcDNA 3.1-TFPI2 and shTWIST1 had no significant effect on cell migration of MCF7 cells compared to cells cotransfected pcDNA 3.1-NC with shTWIST1. k Cotransfection with pcDNA 3.1-TFPI2 and shTWIST1 had no significant effect on cell invasion of MCF7 cells compared to cells cotransfected pcDNA 3.1-NC with shTWIST1