Fig. 1
From: circKIF4A promotes tumorogenesis of glioma by targeting miR-139-3p to activate Wnt5a signaling
Expression of circKIF4A, miR-139-3p and Wnt5a in several glioma cell lines and tissues. The expression level of circKIF4A (a) miR-139-3p (b) and Wnt5a (c) was detected by qRT-PCR in glioma tissues and adjacent normal tissues. The correlation between circKIF4A and miR-139-3p (d), circKIF4A and Wnt5a (e), miR-139-3p and Wnt5a (f) expression were determined by Spearman correlation analysis. g The protein level of Wnt5a was detected by immunohistochemical in glioma tissues and adjacent normal tissues. The expression level of circKIF4A (h) miR-139-3p (i) and Wnt5a (j) was detected by qRT-PCR in four glioma cell lines including A172, SHG44, U251 and LN229, as compared with NHA cell line. k The protein level of Wnt5a was detected by Western blotting in glioma cell lines and NHA cell line. l The quantification of Wnt5a relative protein level as a ratio to that of GAPDH (internal control). *p < 0.05, **p < 0.01, ***p < 0.001