Fig. 2

circKIF4A directly targeted miR-139-3p to promote the expression of Wnt5a in A172 and LN229 cells. a The expression level of circKIF4A was detected by qRT-PCR in A172 and LN229 cells transfected with sh-circKIF4A-1, sh-circKIF4A-2 and sh-circKIF4A-3. b miR-139-3p and Wnt5a were detected by qRT-PCR in A172 and LN229 cells transfected with sh-circKIF4A-2. c The expression level of miR-139-3p and Wnt5a were detected by qRT-PCR in A172 and LN229 cells transfected with miR-139-3p mimics or inhibitor. d Schematic representation of binding sites between circKIF4A and miR-139-3p predicted by StarBase software. e Schematic representation of binding sites between miR-139-3p and Wnt5a predicted by StarBase software. f Luciferase activity assay was performed after co-transfection with circKIF4A reporter plasmid and miR-139-3p into A172 and LN229 cells. g Luciferase activity assay was performed after co-transfection with Wnt5a reporter plasmid and miR-139-3p into A172 and LN229 cells. *p < 0.05, **p < 0.01, ***p < 0.001