Fig. 6

circKIF4A knockdown suppressed the spheroid formation of GICs through miR-139-3p/Wnt5a signaling. After transfecting GICs with sh-circKIF4A, miR-139-3p mimics or miR-139-3p inhibitors, a the sphere growth of GICs was determined by sphere formation assays. b Quantitative analysis of the GICs sphere number. c The protein expression of Wnt5a, GSK-3β, β-catenin and p-β-catenin were detected by Western blotting. d The quantification of Wnt5a, GSK-3β, β-catenin and p-β-catenin relative protein level as a ratio to that of GAPDH (internal control). e The protein expression of c-Myc, cyclin D1, Bcl2 and Bax were detected by Western blotting. f The quantification of c-Myc, cyclin D1, Bcl2 and Bax relative protein level as a ratio to that of GAPDH (internal control).*p < 0.05, **p < 0.01, ***p < 0.001