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Fig. 3 | Molecular Medicine

Fig. 3

From: Estrogen-regulated PTTG1 promotes breast cancer progression by regulating cyclin kinase expression

Fig. 3

PTTG1 expression was regulated by the levels of estrogen. a-b qPCR was employed to detect the mRNA levels of PTTG1 in MCF7 cells in the presence or absence of estrogen (100 nM). c-d shRNA was used to knock down the ESR1 and qPCR was applied to examine the mRNA levels of ESR1 and PTTG1 in the cells. e ER binding sites were predicted using rVista (https://rvista.dcode.org/) and two possible binding sites were found. f Mutations were introduced in the ER binding sites and luciferase reporter assay was performed to confirm the ER binding sites on the promoter on the PTTG1.MCF7 cells were co-transfected with plasmids and vehicles or estrogen. n = 3 for all experiments. Data were represented as mean ± S.D. **P < 0.01, ***P < 0.001, ns indicates no significance

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