Skip to main content
Fig. 1 | Molecular Medicine

Fig. 1

From: Key role of MIF-related neuroinflammation in neurodegeneration and cognitive impairment in Alzheimer’s disease

Fig. 1

In-vitro results of STZ stimulation on murine Microglia and Astrocytes. a ELISA of MIF secretion in supernatants of astrocytes and microglia after 24 h STZ treatment. Graphs represent the mean of n = 3 biological replicates (two technical replicates for each) with standard errors of the mean (± SEM), (*P < 0.05, Student’s t test). b and c mRNA expression levels for different cytokine in response to STZ treatment in astrocytes (b) and microglia (c) with and without ISO-1 treatment. d- f. Cytokine ELISA of astrocytes after 24 h STZ treatment. Data are means of n = 3 independent biological replicates, error bars represents ± SEM (*P < 0.05, Student’s t test). IL-6 (d), IL-1β (e) and IL-12p40 (f) were released in response to STZ treatment in astrocytes using different concentrations of ISO-1 to inhibit MIF resulted in dose dependent decrease in cytokine release in both cell types. g-i Cytokine ELISA in wild type microglia. IL-6 (g) IL-1β (h) and IL-10 (i) were measured after STZ treatment with and without ISO-1. One-way ANOVA with Tukey’s multiple comparison test was performed. (*P < 0.05, **P < 0.01)

Back to article page