| | Control (n = 8) | HFD (n = 8) | HFD + β3-AGO (n = 8) | HFD + β3-ANT (n = 8) |
|---|
Steatosis |
Grade 0 | 8 | 0 | 1 | 0 |
Grade 1 | 35 | 7 | 22 | 1 |
Grade 2 | 5 | 23 | 19 | 17 |
Grade 3 | 0 | 18 | 6 | 30 |
Ballooning |
Grade 0 | 48 | 7 | 19 | 0 |
Grade 1 | 0 | 23 | 29 | 18 |
Grade 2 | 0 | 18 | 0 | 30 |
Inflammation |
Grade 0 | 34 | 0 | 12 | 0 |
Grade 1 | 14 | 6 | 24 | 0 |
Grade 2 | 0 | 31 | 12 | 18 |
Grade 3 | 0 | 11 | 0 | 30 |
- Two sections of each liver specimen were observed and three views of each section were analysed. Therefore, each group had 48 views (8 rats × 2 sections × 3 views) in total. We calculated the view number distributed in grading NAFLD activity scores (NASs) according to the following categories: steatosis (0–3), lobular inflammation (0–3) and ballooning degeneration (0–2). For steatosis, lobular inflammation and ballooning degeneration, P < 0.05, HFD vs control; P < 0.05, HFD vs HFD + β3-AGO; P < 0.05, HFD vs HFD + β3-ANT
