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Fig. 3 | Molecular Medicine

Fig. 3

From: MicroRNA-214 modulates the senescence of vascular smooth muscle cells in carotid artery stenosis

Fig. 3

Cellular and exosomal microRNA-214-3p levels (miR-214) in response to CoCl2. a Mean normalized expression levels of A7r5 cellular miRNAs (miR-19a, −20a, −26b, −106b, − 126, − 214, and − 374) as determined by miRNA-specific SYBR Green PCR assays in response to CoCl2 for 48 h and normalized to RNU6B. Expression values are presented relative to medium only control (n = 6). b Total exosome isolation reagent isolated from A7r5 VSMCs after 48 h at CoC2 were spiked with cel miR-54 and used for RNA extractions. This exosomal RNA was then assayed for miR-214 and cel miR-39 by qRT-PCR and normalized to exogenous cel miR-54 (n = 6). Data are presented as the means ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; ns, non-significance (one-way ANOVA with Tukey-Kramer post hoc test)

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