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Fig. 5 | Molecular Medicine

Fig. 5

From: Overexpression of RPN2 suppresses radiosensitivity of glioma cells by activating STAT3 signal transduction

Fig. 5

RPN2 mediates MCL1 expression by activate STAT3. a The expression of p-STAT3 and STAT3 in U87 parental, resistant cells, or U87 parental cells with RPN2 overexpression. b The immunofluorescence staining of STAT3 in U87 cells transfected with RPN2 plasmid. Left, the representative pictures; right, the nuclear translocation of STAT3 was analyzed by Image J. c U87 resistant cells transfected with STAT3 siRNA were treated with 5 GY radiation. The expression of RNP2, MCL1, STAT3, and CASP3 was analyzed by western blot. d The mRNA level of MCL1 in U87 and A172 resistant cells transfected with STAT3 siRNA. e The apoptosis of U87 and A172 resistant cells treated as in (c). f The IHC staining of p-STAT3 in primary and recurrence GBM tumors. Left, the representative pictures; right, the histological analysis of p-STAT3 expression by Image J. Each experiment was performed for 3 times. *, p < 0.05; **, p < 0.01

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Molecular Medicine

ISSN: 1528-3658