Fig. 1
From: The SP1-12LOX axis promotes chemoresistance and metastasis of ovarian cancer
DDP-resistant ovarian cancer cells possess enhanced metastatic activity and arachidonic acid metabolism. a The procedure for the induction of DDP-resistant SKOV3 cells (upper panel); IC50 and the cell viabilities of SKOV3 and SKOV3-R with different concentrations of DDP treatment (lower panel). b, c The mRNA (b) and protein (c) levels of MRP1, MRP4, Slug and Snail in SKOV3 and SKOV3-R. d Liver metastasis in nude mice after SKOV3 or SKOV3-R subcutaneous transplantation. e, f. Migration and invasion of SKOV3 and SKOV3-R were tested by wound-healing (e) and transwell assays (f). g MRM chromatograms of AA, TXB2, 12-HETE, 5,6-EET, and 14,15-EET. h The supernatant levels of LTB4, 5-HETE, 12-HETE, PGE2, TXB2, 5,6-EET, 14,15-EET, 14,15-DHET, 11-HETE, and 11,12-DHET in SKOV3 and SKOV3-R. i The heatmap for gene expression of 12LOX, COX1, CYP2J2, COX2, 5LOX, 15LOX, LTA4H and PGDH. j the protein levels of 12LOX and COX1 were assessed by western blotting. Data are expressed as mean ± SEM of 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001