Fig. 3

Inhibition of SP1 or 12LOX dampens metastasis and DDP-resistance of SKOV3-R cells in vitro. a-d After incubation with Bai (20 μM) or MA (30 nM) for 24 h, migration and invasion were tested by wound-healing assays (a) and transwell assays (b); the cell viability percentages were measured by CCK-8 assay (c, d). siNC denoted SP1 siRNA negative control, si-SP1 denoted SP1 siRNA. e-g After SKOV3-R cells were treated with these siRNAs, the gene expression levels of SP1 was evaluated by qPCR (e) and migration and invasion were tested by wound-healing assays (f) and transwell assays (g). h-j After administration with Bai (20 μM) or MA (30 nM) for 24 h, the heatmap for gene expression of MRP1, MRP4, SP1, 12LOX, Slug and Snail (h) and the protein level of MRP1, MRP4, Slug and Snail were evaluated by western blotting (i,j). Data are expressed as mean ± SEM of 4 independent experiments. ^***P < 0.001