Fig. 4

Blocking SP1 or 12LOX attenuates SKOV3-R tumor growth and reverses metastasis in vivo. a SKOV3-R cells (1 × 10⁶) were subcutaneously injected in nude mice, tumor reached ~ 300 mm³ at Day 30, then the mice were treated with PBS or DDP (1 mg/kg) or DDP (1 mg/kg) plus Bai (30 mg/kg) or MA (0.5 mg/kg) once every 2 days. The tumor volume was assessed until Day 36. b SKOV3-R cells (1 × 10⁶) were subcutaneously injected in nude mice. The mice were treated with PBS or DDP (1 mg/kg) plus Bai (30 mg/kg) or MA (0.5 mg/kg) every 2 days in the third week. Mice were sacrificed for assessment of liver metastasis at Day 21. The overall view of the livers, H&E staining and the metastasis numbers in live lesions (right panel) were determined. Data in (b) are expressed as mean ± SEM for 5 mice. c IHC of SP1, 12LOX, MRP1 and Snail in liver tissues in four groups including control (PBS), DDP, DDP plus MA, DDP plus Bai. IOD against control group in above groups were depicted in fold change. d The correlation between SP1, 12LOX, COX1, CYP2J2 with MRPs, Slug and Snail. Pearson correlation coefficient was used to analyze the correlation between MRPs and EMT markers with SP1, 12LOX, COX1, and CYP2J2 using the clinical database GSE13876. e Schematic diagram of SP1-driven chemoresistance and metastasis. Data are expressed as mean ± SEM for 5 mice. ^*P < 0.5, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001; DDP + MA vs. DDP; ^#P < 0.05, DDP + Bai vs. DDP