Fig. 1

Gram-negative pneumonia results in murine acute lung injury with changes in lung collagen content and localization of collagen-producing cells. a Bronchoalveolar lavage (BAL) albumin concentration on day 3 of acute lung injury caused by experimental Klebsiella pneumonia. Each dot represents one animal; mean and SEM are indicated. b Change in lung soluble collagen after onset of acute lung injury; n = 4–6 experimental animals per time point per group. c Initial panel at low power magnification (scale bar = 50 μm) demonstrates lung immunofluorescent staining of normal (uninfected) lung tissue showing staining for CD45, N-terminal pro-collagen I (proCol1), collagen III (Col3), α-smooth muscle actin (α-SMA), and nuclear stain (DAPI). Subsequent panels show high-power magnification demonstrate representative images on day 3 of lung injury. *, p < 0.05 by Mann-Whitney test (panel A) and 2-way ANOVA (panel B)