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Fig. 3 | Molecular Medicine

Fig. 3

From: The α7 nicotinic acetylcholine receptor agonist, GTS-21, attenuates hyperoxia-induced acute inflammatory lung injury by alleviating the accumulation of HMGB1 in the airways and the circulation

Fig. 3

Systemic administration of GTS-21 inhibits infiltration of neutrophils and monocytes into the airways of mice exposed to hyperoxia. C57BL/6 mice were exposed to room air (21% O2) or hyperoxia (≥99% O2) and treated with vehicle (0 mg/kg) or GTS-21 (4 mg/kg), as described in Fig. 1. a The total amount of leukocytes in the BAL were determined by hemocytometer. b Cells from the BAL samples were analyzed using differential cell staining. Arrows show the presence of neutrophils stained with eosin and methylene blue in BAL samples. c The total number of infiltrated neutrophils in the BAL samples. d-f Cells from BAL were labeled with anti-CD11b and anti-CD11c antibodies and analyzed by flow cytometry (d-e); MCP-1 were analyzed by ELISA (f). d Representative gating for CD11b+hi and CD11clow cells in the BAL. e The graph is a representative of two independent experiments. f The bar graph for MCP-1 expression in BAL. Data is represented as the mean ± SEM (n = 2–6 per group). *p < 0.05, compared with mice receiving normal saline

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Molecular Medicine

ISSN: 1528-3658