Fig. 4

iNOS inhibition reversed the iNOS/NO induced signaling. a Human hepatocytes were treated with L-NIL (100 μM, 24 h), and IRF1 (nuclear extracts) and PUMA (whole cell extracts) were analyzed by Western blot. b Similar to (a), but representative images of immunofluorescence staining are shown, red: IRF1; green: F-actin; blue: nucleus. c Mouse hepatocytes were transfected with IRF1-luciferase reporter for 24 h, and followed the treatments as indicated for 9 h. Luciferase reporter assay was performed. L-NIL decreased IFNγ-induced IRF1 transcriptional response, *P = 0.03. The data shown are representative of three experiments with similar results. d Warm I/R mice (n = 4) were used for the study of iNOS inhibition reducing liver injury. Ischemia was performed for 1 h, and then reperfusion with the treatment of BYK191023 (60 mg/kg, 6 h). TUNEL staining of apoptotic cells with green color on the liver tissues (left) and ALT concentrations (right) were reduced by BYK191023 vs. the controls, **P = 0.003