Fig. 5
From: iNOS/NO is required for IRF1 activation in response to liver ischemia-reperfusion in mice
IRF-1 synergistically targets PUMA gene expression with p53. a HCT116TP53+/+ and HCT116TP53−/− cells were treated with IFNγ (24 h). Immunofluorescence staining was performed with the indicated antibodies. Representative images are shown, red: IRF1; green: PUMA. b 293 T cells were transfected with pCMV6-xl5-hIRF1 (3 μg) or pCMV-xl5-TP53 (1 μg); and co-transfected with pCMV6-xl5-hIRF1 (3 μg) and pCMV-xl5-TP53 (1 μg) for 24 h. Total proteins were analyzed by Western blot with the indicated antibodies