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Fig. 6 | Molecular Medicine

Fig. 6

From: iNOS/NO is required for IRF1 activation in response to liver ischemia-reperfusion in mice

Fig. 6

iNOS/NO was required for HDAC2 activity which up-regulated IRF1 nuclear translocation. a Mouse (left) and human (right) hepatocytes were treated with GSNO (1 μM) or SNAP (500 μM) for 3 h, respectively. Nuclear expressions of IRF1, HDAC2 and H3AcK9 were analyzed by Western blot; lamin A/C was loading controls. b Mouse hepatic I/R were performed 1 h ischemia and a various times of reperfusion as indicated. The nuclear expressions of HDAC2 and H3AcK9 in liver tissues were measured by Western blot. c 293 T cells were treated with GSNO (1 μM) and romidepsin (5 μM) for 3 h. The expressions of IRF1, HDAC2 and H3AcK9 (nuclear extracts), and PUMA and p21 (whole cell extracts) were analyzed by Western blot

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