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Fig. 2 | Molecular Medicine

Fig. 2

From: RETRACTED ARTICLE: WRN inhibits oxidative stress-induced apoptosis of human lensepithelial cells through ATM/p53 signaling pathway and its expression is downregulated by DNA methylation

Fig. 2

WRN overexpression suppresses apoptosis and oxidative stress of SRA01/04 cells in vitro. SRA01/04 cells were treated with different concentrations (0, 25, 50 and 100 μM) of H2O2. a QRT-PCR and (b) WB were performed to estimate the gene and protein expression of WRN in the SRA01/04 cells. c MSP was performed to detect the methylation level of WRN in the SRA01/04 cells. SRA01/04 cells were transfected with pcDNA3.1-WRN or pcDNA3.1-NC, and then the modified SRA01/04 cells were treated with 100 μM H2O2. d CCK8 was performed to explore the cell proliferation of the modified SRA01/04 cells. e The apoptosis of the modified SRA01/04 cells was determined by flow cytometry. f The content of ROS in the modified SRA01/04 cells was detected by fluorescent probe DCFH-DA. g WB was performed to detect the expression of SOD, CAT and MDA in the modified SRA01/04 cells. M: methylation; U: unmethylation. (*P < 0.05 compared with the 0 μM H2O2 or Control group, **P < 0.01 compared with the 0 μM H2O2 or Control group, #P < 0.05 compared with the H2O2 + Vector group, ##P < 0.01 compared with the H2O2 + Vector group)

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Molecular Medicine

ISSN: 1528-3658