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Fig. 3 | Molecular Medicine

Fig. 3

From: RETRACTED ARTICLE: WRN inhibits oxidative stress-induced apoptosis of human lensepithelial cells through ATM/p53 signaling pathway and its expression is downregulated by DNA methylation

Fig. 3

5-Aza-CdR treatment suppresses apoptosis and oxidative stress of SRA01/04 cells in vitro. SRA01/04 cells were consecutively treated with 100 μM H2O2 and 5-Aza-CdR. Normal SRA01/04 cells served as control. a QRT-PCR and (b) WB were performed to detect the gene and protein expression of WRN in the SRA01/04 cells. c CCK8 was performed to explore the cell proliferation of the SRA01/04 cells. d The apoptosis of the SRA01/04 cells was determined by flow cytometry. e The content of ROS in the SRA01/04 cells was detected by fluorescent probe DCFH-DA. f WB was performed to explore the expression of SOD, CAT and MDA in the SRA01/04 cells. (**P < 0.01 compared with the Control group, ##P < 0.01 compared with the H2O2 group)

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Molecular Medicine

ISSN: 1528-3658