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Fig. 4 | Molecular Medicine

Fig. 4

From: RETRACTED ARTICLE: WRN inhibits oxidative stress-induced apoptosis of human lensepithelial cells through ATM/p53 signaling pathway and its expression is downregulated by DNA methylation

Fig. 4

5-Aza-CdR treatment suppresses apoptosis and oxidative stress of SRA01/04 cells by promoting WRN expression in vitro. SRA01/04 cells were transfected with WRN siRNA or Scramble. Normal SRA01/04 cells served as control. a QRT-PCR and (b) WB were performed to detect the gene and protein expression of WRN in the modified SRA01/04 cells. The normal and modified SRA01/04 cells were consecutively treated with 100 μM H2O2 and 5-Aza-CdR. c QRT-PCR and d WB were performed to detect the gene and protein expression of WRN in the modified SRA01/04 cells. e CCK8 was performed to explore the cell proliferation of the modified SRA01/04 cells. f The apoptosis of the modified SRA01/04 cells was determined by flow cytometry. g The content of ROS in the modified SRA01/04 cells was detected by fluorescent probe DCFH-DA. h WB was performed to detect the expression of SOD, CAT and MDA in the modified SRA01/04 cells. (**P < 0.01 compared with the Scramble group, ##P < 0.01 compared with the Control or Control+Scramble group, $$P < 0.01 compared with the Control+WRN siRNA group)

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Molecular Medicine

ISSN: 1528-3658