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Fig. 6 | Molecular Medicine

Fig. 6

From: RETRACTED ARTICLE: WRN inhibits oxidative stress-induced apoptosis of human lensepithelial cells through ATM/p53 signaling pathway and its expression is downregulated by DNA methylation

Fig. 6

WRN overexpression inhibits apoptosis and oxidative stress of SRA01/04 cells via ATM/p53 signaling pathway. SRA01/04 cells were transfected with pcDNA3.1-WRN or pcDNA3.1-NC, and then the modified SRA01/04 cells were treated with 50 μM chloroquine. a WB was performed to detect the expression of p-ATM, ATM, p53, p-p53, p21, Bax, PUMA and NOXA in the modified SRA01/04 cells. b CCK8 was performed to explore the cell proliferation of the modified SRA01/04 cells. c The apoptosis of the modified SRA01/04 cells was determined by flow cytometry. d The content of ROS in the modified SRA01/04 cells was detected by fluorescent probe DCFH-DA. e WB was performed to explore the expression of SOD, CAT and MDA in the modified SRA01/04 cells. (*P < 0.05 compared with the Vector group, **P < 0.01 compared with the Vector group, #P < 0.05 compared with the Vector+Chl group, ##P < 0.01 compared with the Vector+Chl group)

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Molecular Medicine

ISSN: 1528-3658