Fig. 1From: Caspase1/11 signaling affects muscle regeneration and recovery following ischemia, and can be modulated by chloroquineCQ consolidates LC3 expression in ischemic muscle. Immunohistochemical analysis of cross sections of nonischemic and ischemic tibialis anterior (TA) muscle from WT (C57Bl6/J) mice at 24 h after femoral artery ligation (FAL) treated with either PBS or CQ (50 mg/kg) for 21 days prior to unilateral FAL. LC3 expression (red); actin expression (green); DAPI nuclear stain (blue); pattern of LC3 expression (diffuse versus consolidated) analyzed using integrated density analysis (Image J) and expressed as a function of actin per unit area. Five images per limb/animal were evaluated. Representative images shown. *p < 0.005, PBS:CQ, ischemic limb N = 3 mice per treatment, 5 images/limb for each mouse; Scale bar = 10 μmBack to article page