Fig. 3

CQ increases caspase-1 expression in muscle. a Immunofluorescence of tibialis anterior (TA) muscle harvested 21 days after unilateral femoral artery ligation (FAL) in WT (C57Bl6/J) mice treated with PBS or CQ (50 mg/kg). Caspase-1 (red); DAPI nuclear stain (blue). Representative images shown (b) Caspase-1 mean fluorescence intensity (MFI) calculated after color separation using Image J analysis. * P < 0.01, PBS:CQ in WT and KO. c Western blots of cleaved caspase-1 and GAPDH loading control in whole muscle homogenates. Semi-quantitative analysis performed with Image J Gel Analysis of expression of cleaved caspase-1 relative to GAPDH. *p < 0.001 PBS:CQ, N = 4–5 mice per experimental group; Scale bar = 10 μm