Fig. 8

Myocyte fusion index (MFI) is attenuated in ischemia, CQ and absence of caspase-1/11 (a) Immunofluorescence of MF20 (green) and with DAPI (blue) in muscle stellate cells (MuSC) 5 days after harvest from tibialis anterior (TA) of nonischemic and ischemic limbs of C57Bl6/J (WT) and caspase-1/11KO mice at 21 days after FAL treated with either PBS or CQ. (b) Myocyte fusion index (MFI) quantified as number of nuclei within multinucleated fibers (> 3 nuclei) dividing by total number of nuclei. C57Bl6/J (WT) (white bars) and caspase-1/11KO (black bars) * p < 0.05, **p = 0.01, ***p < 0.001; N = 4 mice per strain per treatment group; Scale bar = 10 μm