Fig. 2

circCELSR1 sponged miR-598 to stimulate BRD4 gene expression in SKOV3 and A2780 cells. a Gene expression of circCELSR1, miR-598 and BRD4 were detected by qRT-PCR in SKOV3 and A2780 cells transfected with sh-circCELSR1 or shNC. Control group represented cells without any treatment. b Gene expression of miR-598 and BRD4 were detected by qRT-PCR in SKOV3 and A2780 cells transfected with miR-598 mimics and miR-598 inhibitor. Control group represented cells without any treatment. Bioinformatics prediction of the binding site between circCELSR1 and miR-598 (c) and between miR-598 and BRD4 3′-UTR (d). Luciferase activity assay was performed after co-transfection with circCELSR1 reporter plasmid and miR-598 into SKOV3 and A2780 cells. f Luciferase activity assay was performed after co-transfection with BRD4 reporter plasmid and miR-598 into SKOV3 and A2780 cells. All the results were shown as mean ± SD (n = 3). * p < 0.05 and ** p < 0.01