Fig. 1
From: THRIL mediates endothelial progenitor cells autophagy via AKT pathway and FUS
LncRNA THRIL induced cell autophagy and promotes CAD progression. (a) THRIL was overexpressed in CAD blood samples and EPCs. *P < 0.05, compared with normal (healthy) group. The number of CAD patients and healthy controls were all twenty. (c) THRIL was depressed in EPCs in transfection with sh-THRIL-1 or sh-THRIL-2 and overexpressed through using pCMV6-THRIL. The RNA expression of THRIL was measured through qRT-PCR. *P < 0.05, compared with NC group. (c) Cell viability was measured through using CCK-8 assays. (d) Flow cytometry results revealed that sh-THRIL reduced apoptotic cell number of EPCs. (f) Autophagy assays results revealed that sh-THRIL reduced EPCs autophagy rate. Normal: EPCs from healthy controls; NC: EPCs isolated from CAD patients; All the transfection experiments were performed in EPCs isolated from CAD patients. *P < 0.05, compared with NC group; #P < 0.01, compared with normal ones. The data shown represent three separate experiments performed in triplicate in each experiment (mean ± the standard deviation [SD])