Fig. 3

Secretion of resistin from cord blood mononuclear cells in response to metabolic stresses. a Expression of resistin mRNA in placenta and cord blood mononuclear cells (CBMCs), cord tissue mesenchymal stem cells (MSC) of healthy subjects (N = 5 subjects for placental tissues and CBMCs; N = 3 subjects for MSCs), and BeWo cells (trophoblast cell line) were measured by quantitative RT-PCR; b Mononuclear cells were isolated from cord blood of healthy pregnant women and were treated with TNFα (100 ng/ml), high glucose (25 mM), palmitate acid (0.6 mM), or 4-Hydroxynonenal (4-HNE, 0.6 mM) for 16 h, followed by measuring resistin levels in culture media with ELISA. M: mannitol treated group as an osmotic control for high group treatment. Mean ± SD, ** P < 0.01; *** P < 0.001; N = 4