Fig. 4

FBXW7 is the direct target of miR-194. a The predicted binding sequence of the 3’UTR of FBXW7’s mRNA and miR-194. b The relative luciferase activity was significantly reduced in DRG neurons co-transfected with wt-lincRNA PADNA vector and miR-194 mimics than in DRG neurons co-transfected with mut-lincRNA PADNA vector or empty vector or mimic NC. c miR-194 negatively regulate the expression of FBXW7. d The expression of FBXW7 protein was significantly decreased in DRG neurons treated with miR-194 than in DRG neurons treated with mimic NC; while the expression of FBXW7 protein was significantly increased in DRG neurons treated miR-194 inhibitors than in DRG neurons treated with inhibitor NC. e RT-PCR revealed the expression of FBXW7 mRNA is negatively correlated with the expression of miR-194. f The expression of FBXW7 mRNA was significantly upregulated with the elevation of concentration of bupivacaine. g The expression of FBXW7 mRNA was significantly upregulated with the extension of bupivacaine treatment. Cells were treated with 1 mM bupivacaine and expression of FBXW7 was measured under different time points. Data are Mean ± SD with n = 3 independent biological cultures. *p < 0.05 and ** p < 0.01