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Fig. 7 | Molecular Medicine

Fig. 7

From: SHP2 inhibitor PHPS1 ameliorates acute kidney injury by Erk1/2-STAT3 signaling in a combined murine hemorrhage followed by septic challenge model

Fig. 7

Effects of PHPS1 on expression of activation of Erk1/2 and STAT3 in kidney after Hem/CLP. Twenty four h post Hem/CLP or sham operation, the kidneys were collected and tissue homogenates were obtained. The extent of Erk1/2 (a) and STAT3 (b) activation (phosphorylation) was determined by western blot. The ratio of phosphorylated Erk1/2 and total Erk1/2 (p-Erk1/2/t-Erk1/2) and phosphorylated STAT-3 and total STAT-3 (p-STAT-3 /t-STAT-3) in kidneys from Hem/CLP mice was significantly increased compared to Shams. PHPS1 treated Hem/CLP mice showed reduced Erk1/2 and STAT3 activation compared to Veh treated HEM/CLP mice. *, P < 0.05, versus sham (S/S); #, P < 0.05, PHPS1 treated group (Hem/CLP + PHPS1) versus Hem/CLP + vehicle (Hem/CLP). One-way ANOVA and a Student-Newman-Keuls’ test, Mean ± SD; n = 6 mice/group

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