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Fig. 1 | Molecular Medicine

Fig. 1

From: SP1 induced long non-coding RNA AGAP2-AS1 promotes cholangiocarcinoma proliferation via silencing of CDKN1A

Fig. 1

SP1 induces high AGAP2-AS1 expression in CCA. a, b Relative expression of AGAP2-AS1 in CCA tissues compared with the corresponding adjacent normal tissues (n = 32), and classification into two groups. c Kaplan–Meier overall survival curves based on AGAP2-AS1 expression level. d Analysis of AGAP2-AS1 expression levels in CCA cell lines (HUCCT1 and RBE) by qPCR. e, f qRT-PCR analysis of AGAP2-AS1 expression of HUCCT1 and RBE cells with si-AGAP2-AS1 1, 2, pcDNA-AGAP2-AS1, or the negative control. g JASPR prediction of SP1 binding sites in the AGAP2-AS1 promoter regions. h SP1 knockdown and overexpression efficiency. i Effect of knockdown or overexpression of SP1 on AGAP2-AS1. j CHIP for SP1 in the promoter region of AGAP2-AS1. k Luciferase reporter gene verification. Representative images and data based on three independent experiments. Bars: ± sd, *P < 0.05, **P < 0.01

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