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Fig. 2 | Molecular Medicine

Fig. 2

From: SP1 induced long non-coding RNA AGAP2-AS1 promotes cholangiocarcinoma proliferation via silencing of CDKN1A

Fig. 2

AGAP2-AS1 promotes CCA cell proliferation in vitro and vivo. a, b CCK-8 assay was performed to determine the proliferation of HUCCT1 and RBE cells with si-AGAP2-AS1 1, 2, pcDNA-AGAP2-AS1, or the negative control. c Colony formation assays were performed to determine the proliferation of sh-AGAP2-AS1-transfected HUCCT1 and RBE cells. d The percentage of apoptotic cells was determined by flow cytometric analysis. e Stable AGAP2-AS1 knockdown were used for the in vivo study. Nude mice carrying tumors from respective groups were shown. f Tumor volumes were calculated every 3 days after injection. g Tumor weights from two groups are represented. h qPCR was performed to detect the average expression of AGAP2-AS1 in xenograft tumors. i Images of HE staining and immunohistochemistry in xenograft tumors. Representative Ki-67 protein levels in xenograft tumors as evaluated by IHC. Representative images and data based on three independent experiments. Bars: ± sd, *P < 0.05, **P < 0.01

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