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Fig. 1 | Molecular Medicine

Fig. 1

From: The α7 nicotinic acetylcholine receptor agonist GTS-21 improves bacterial clearance in mice by restoring hyperoxia-compromised macrophage function

Fig. 1

Systemic administration of GTS-21 increases bacterial clearance and decreases acute lung injury in mice exposed to hyperoxia and challenged with PA. C57BL/6 mice were exposed to ≥ 99% O2 for 48 h and then inoculated with PA (0.1 × 108 CFUs/mouse), and returned to 21% O2 after inoculation. Mice were randomized to receive either GTS-21 (0.04, 0.4, and 4 mg/kg) or saline, administrated by intraperitoneal injection, every 8 h starting at 32 h during hyperoxia. BAL and lung tissue were harvested 18 h after inoculation. Viable bacteria in the airways and lungs were quantified by plating serial dilutions of homogenized lung (a) and BAL (b), and are expressed as log CFUs/lung and log CFUs/ml of BAL, respectively. The total protein content in the BAL (c) was measured as a marker of lung injury. Data represent the means ± SEMs from two independent experiments (n = 5 for control, n = 6 for all GTS-21 treated mice). *p < 0.05, compared with mice receiving normal saline

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