Fig. 2
GTS-21 restores hyperoxia-compromised phagocytic activity of macrophages. RAW 264.7 cells were either exposed to 95% O2 alone (black bar) or 95% O2 in the presence of different concentrations of GTS-21 (grey bars) or remained at 21% O2 (white bar) for 24 h. Cells were then incubated with FITC-labeled latex minibeads for 1 h and stained with phalloidin to visualize the cells. a Immunofluorescent images of RAW 264.7 cells (Red: actin cytoskeleton; Green: minibeads). At least 250 cells per well from 3 independent experiments were counted and the number of beads per cell was represented as percent beads phagocytosed in the bar graph (b). Each value represents mean ± SEM of three independent experiments for each group. *p < 0.05 and **p < 0.01 versus 95% O2 alone (black bar). Bar, 10 µm