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Fig. 3 | Molecular Medicine

Fig. 3

From: The α7 nicotinic acetylcholine receptor agonist GTS-21 improves bacterial clearance in mice by restoring hyperoxia-compromised macrophage function

Fig. 3

GTS-21 inhibits the accumulation of extracellular HMGB1. a RAW 264.7 cells were either exposed to 95% O2 alone (black bar) or 95% O2 in the presence of a series of concentrations of GTS-21 (grey bars) or remained at 21% O2 (white bar) for 24 h. HMGB1 levels in cell culture media were analyzed by Western blot analysis. Representative image of the immunoreactive bands on Western blots is shown. Bar graph shows the integrated density value of HMGB1 bands. The data are expressed as mean ± SEM of three independent experiments. **p < 0.01 versus control 95% O2 alone (black bar). b C57BL/6 mice were exposed to ≥ 99% O2 for 48 h and then inoculated with PA (0.1 × 108 CFUs/mouse), and returned to 21% O2 after inoculation. Mice were randomized to receive either GTS-21 or saline, administrated by intraperitoneal injection every 8 h, starting at 32 h during hyperoxia. Representative image of Western blot immunoreactive bands of HMGB1 in the BAL of these mice is shown. Bar graph shows the integrated density value of HMGB1 bands in BAL of mice that received saline (black bar, n = 5) and GTS-21 (4 mg/kg, grey bar, n = 6)

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