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Fig. 3 | Molecular Medicine

Fig. 3

From: Downregulation of the ubiquitin ligase KBTBD8 prevented epithelial ovarian cancer progression

Fig. 3

Human KBTBD8 was important for the proliferation of EOC cells. a, b The colony number in K8-OE group was significantly more than control group, whereas the colony number in K8-KD-OE group was more than K8-KD group while close to control group (Colony number, CTR vs. K8-KD vs. K8-KD-OE vs. K8-OE, 130 vs. 48 vs. 127 vs. 157). ce Cell cycle analysis by FACS showed that significantly fewer cells were at the G2 stage (proportion of cells at G2, CTR vs. K8-KD, 29.22% vs 10.09%) and more cells were at the G1 stage (percentage of cells at G1, CTR vs. K8-KD, 45.72% vs. 67.39%) in KBTBD8-knockdown A2780 cells than in control cells. PI, Propidium Iodide. f CCK8 assay showed that there were less proliferating cells in the KBTBD8-knockdown A2780 cells than in control cells (CCK8, CTR vs. K8-KD, 0.7672 vs. 0.5211). A.U., arbitrary unit. Different characters above the column between groups indicate significant difference. **Indicates p < 0.01, ****indicates p < 0.0001

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