Fig. 4

Human KBTBD8 was important for the migration of EOC cells. a, b Wound healing assays showed that the closure ratio in K8-OE group was more than in control group; whereas the closure ratio in K8-KD-OE group was more than K8-KD group while close to control group. Representative images at 0 h, 24 h, 48 h, 72 h, and 96 h were shown. Percentage of initial scratched area uncovered by migrated cells at 24 h, CTR vs. K8-KD vs. K8-KD-OE vs. K8-OE, 72.99% vs. 90.38% vs. 74.58% vs. 82.79%; at 48 h, 62.95% vs. 77.26% vs. 57.82% vs. 59.50%; at 72 h, 30.29% vs. 57.35% vs. 45.35% vs. 33.13%; at 96 h, 10.90% vs. 43.61% vs. 15.54% vs. 8.896%. c, d 3-dimensional migration assay showed that the number of migrated cells in K8-OE group was more than in control group, whereas the number of migrated cells in K8-KD-OE group was more than in K8-KD group while close to control group (Number of migrated cells per field, CTR vs. K8-KD vs. K8-KD-OE vs. K8-OE, 73 vs. 19 vs. 62 vs. 160). Scale bar, 50 μm. Different characters above the column between groups indicate significant difference