Fig. 5
From: Downregulation of the ubiquitin ligase KBTBD8 prevented epithelial ovarian cancer progression
Human KBTBD8 was important for the general health state of EOC cells. a, b KBTBD8 knockdown significantly increased ROS level in A2780 ovarian cancer cells with or without NAC (ROS level without NAC, CTR vs. K8-KD, 1.458 vs. 9.313; ROS level with NAC, CTR vs. K8-KD, 1.125 vs. 4.125). c, d JC-1 staining assay showed that the mitochondria membrane potential significantly decreased after the KBTBD8 knockdown (Aggregate: monomer, CTR vs. K8-KD, 4.580 vs. 0.3118). Aggregate in green, monomer in red. e, f Annexin V staining assay showed that KBTBD8 knockdown significantly increased the apoptosis level (Annexin V level, CTR vs. K8-KD, 1.183 vs. 4.775). Annexin V signal in green, PI in red. A.U., arbitrary unit. Scale bar, 50 μm. Different characters above the column between groups indicate significant difference. ***Indicates p < 0.01, ****indicates p < 0.0001