Skip to main content

Table 2 Relative gene expression of hepatic CPT-1, MCAD, LCAD and VLCAD in WT and HC-HMGB1-KO mice on LFD or HFD for 8 weeks

From: Hepatocyte high-mobility group box 1 protects against steatosis and cellular stress during high fat diet feeding

Gene mRNA relative expression
LFD HFD
WT HC-HMGB1−/− P value WT HC-HMGB1−/− P
value
β-oxidation
 CPT-1α 1.24 ± 0.46 0.63 ± 0.24 0.069 0.98 ± 0.14 0.56 ± 0.18 0.010
 MCAD 1.08 ± 0.35 0.57 ± 0.15 0.052 1.26 ± 0.20 0.48 ± 0.07 0.002
 LCAD 0.97 ± 0.14 0.91 ± 0.07 0.450 0.99 ± 0.14 0.52 ± 0.14 0.003
 VLCAD 1.01 ± 0.34 0.49 ± 0.27 0.054 0.91 ± 0.021 0.41 ± 0.0  < 0.001
Lipogenesis
 FAS 0.96 ± 0.05 0.87 ± 0.32 0.610 0.93 ± 0.41 0.78 ± 0.18 0.550
 PPAR-γ 0.96 ± 0.04 0.70 ± 0.24 0.120 1.87 ± 0.66 1.69 ± 0.17 0.630
 SCD-1 1.01 ± 0.29 1.47 ± 0.39 0.110 0.80 ± 0.10 2.32 ± 0.98 0.053
 Adipophilin 0.89 ± 0.21 0.41 ± 0.38 0.091 2.78 ± 1.99 0.44 ± 0.49 0.096
Lipolysis
 LPL 1.18 ± 0.25 0.28 ± 0.30 0.004 0.16 ± 0.15 0.40 ± 0.17 0.079
 SREBP1 1.42 ± 0.21 1.16 ± 0.21 0.130 0.96 ± 0.29 0.62 ± 0.14 0.098
  1. n = 4 for each group; data presented as mean ± SEM