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Fig. 3 | Molecular Medicine

Fig. 3

From: LncRNA LUADT1 sponges miR-195 to prevent cardiac endothelial cell apoptosis in sepsis

Fig. 3

Overexpression of LUADT1 and miR-195 did not affect the expression of each other. HCAECs were transfected with LUADT1 expression vector and miR-195 mimic. LUADT1 and miR-195 overexpression was confirmed by RT-qPCR at 24 h post-transfection (a). The effects of LUADT1 and miR-195 overexpression on the expression of each other were also analyzed by RT-qPCR at 24 h post-transfection (b). The effects of overexpression of LUADT1 and miR-195 mimic on the expression of Pim-1, a downstream target of miR-195, at both mRNA and protein levels were analyzed by RT-qPCR (c) and western blot (d). Experiments were repeated 3 times, and data were expressed a mean value. *, p < 0.05

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