Fig. 4
From: LncRNA LUADT1 sponges miR-195 to prevent cardiac endothelial cell apoptosis in sepsis
LUADT1 suppressed LPS-induced apoptosis of HCAECs through miR-195/Pim-1. HCAECs were cultured in cell culture medium containing 0, 100, 150, 200 and 500 ng/ml LPS for 24 h, followed by the measurement of the expression of LUADT1 by RT-qPCR (a). Cell apoptosis assay was performed to analyze the effects of LUADT1, miR-195 and Pim-1 overexpression on the apoptosis of LUADT1 treated by 150 ng/ml LPS (b). Caspase3 activity assay was detected by Western blotting (c). Apoptosis was detected after the overexpression of Pim-ko and LUADT1 (d). The expression of Pim-1 and apoptosis were examined after using miR-195 antagomir at 150 ng/mL LPS (e). All experiments were repeated 3 times and data were expressed as mean values. *, p < 0.05