Fig. 1

XIST was up-regulated in AML bone marrow cells while its silencing disrupted cell proliferation and enhanced cell apoptosis and Adriamycin sensitivity. a RT-qPCR was used to determine the expression of XIST in normal (n = 20) and AML bone marrow samples (n = 62). b RT-qPCR was used to determine the relative expression of XIST in KG-1 cells. c The subcellular localization of XIST in KG-1 cells identified by FISH assay. Red indicates XIST and blue represents nucleus. d Silencing efficiency of XIST in KG-1 cells assessed by RT-qPCR. e CCK-8 assay was used to detect the proliferation of KG-1 cells. F, The apoptosis of KG-1 cells detected by flow cytometer. g The sensitivity of KG-1 cells to Adriamycin. *p < 0.05 vs. normal bone marrow samples, normal bone marrow cell lines or si-NC-transfected cells. **p < 0.01 vs. si-NC-transfected cells. ***p < 0.001 vs. si-NC-transfected cells. The above data were measurement data, and expressed as mean ± standard deviation. The data between two groups were analyzed by unpaired t-test. The sensitivity of KG-1 cells to Adriamycin detected by CCK-8 assay at different time points was analyzed using two-way ANOVA. The experiment was repeated 3 times independently