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Table 1 Primer sequences for RT-qPCR

From: Silencing long non-coding RNA XIST suppresses drug resistance in acute myeloid leukemia through down-regulation of MYC by elevating microRNA-29a expression

Targeted genes

Forward sequence (5′–3′)

Reverse sequence (5–3′)

XIST

TCCTTTTCTGGCATCAGCGTT

GGCATCACCTCCTGGTTGAAT

miR-29a

TGCGCTAGCACCATCTGAAAT

CAGTGCAGGGTCCGAGGT

MYC

GGCTCCTGGCAAAAGGTCA

CTGCGTAGTTGTGCTGATGT

MRP1

TTCCGGAACTACTGCCTGCGCTA

GGGTCCTGGGGGATGATGGTG

P-GP

CCCATCATTGCAATAGCAGG

GTTCAAACTTCTGCTCCTGA

U6

CTCGCTTCGGCAGCACA

AACGCTTCACGAATTTGCGT

GAPDH

GGAGCGAGATCCCTCCAAAAT

GGCTGTTGTCATACTTCTCATGG

  1. RT-qPCR, reverse transcription quantitative polymerase chain reaction; MRP1, multidrug resistance-associated protein 1, P-gp, P-glycoprotein; Bcl-2, B-cell leukemia/lymphoma 2, GAPDH, glyceraldehyde-3-phosphate dehydrogenase
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Molecular Medicine

ISSN: 1528-3658