Fig. 1
From: LncRNA KCNA2-AS regulates spinal astrocyte activation through STAT3 to affect postherpetic neuralgia
KCNA2-AS was highly expressed in the spinal cord of PHN model rats, and was correlated with GFAP expression. Varicella zoster virus-infected CV-1 cells (monkey kidney cell line), and 5 × 106 cells were injected into rats (PHN group) to construct a PHN rat model. Sham group rats were injected with uninfected CV-1 cells. The experiment lasted 4 weeks, and 5 rats were sacrificed in each group every other week. a Schematic diagram of rat treatment. b Mechanical allodynia was detected by (PWT) before injection or sacrifice in rats. c The mRNA and protein levels of GFAP were detected by qRT-PCR and Western blot. d The expression of five lncRNAs in the spinal cord tissue of rats sacrificed at week 4 was detected by qRT-PCR. e, f The expression levels of KCNA2-AS and H19 were detected by qRT-PCR at different time points. g The correlation between the expression of KCNA2-AS or H19 and GFAP mRNA were analyzed. *P < 0.05 and **P < 0.01 vs. sham group