Fig. 5
From: LncRNA KCNA2-AS regulates spinal astrocyte activation through STAT3 to affect postherpetic neuralgia
Knockdown of KCNA2-AS in the spinal cord of PHN rats alleviates neuropathic pain. Varicella zoster virus-infected CV-1 cells (monkey kidney cell line), and 5 × 106 cells were injected into rats to construct a PHN rat model, and 3 days before, the rats were intrathecal injected (PHN + si-KCNA2-AS group) or no injection (PHN group) of LV-si-KCNA2-AS. The rats were sacrificed after 2 weeks. a Schematic diagram of rat treatment. b Mechanical pain was detected by the PWT method before or 1 and 2 weeks after injection in rats. c The levels of KCNA2-AS were detected in spinal cord tissue by qRT-PCR. d Immunofluorescence staining was performed on the sections of rat spinal cord. e The levels of pSTAT3 and GFAP protein were detected in spinal cord tissue by Western blot. *P < 0.05 and **P < 0.01 vs. PHN group