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Fig. 4 | Molecular Medicine

Fig. 4

From: Downregulation of long noncoding RNA DLEU1 attenuates hypersensitivity in chronic constriction injury-induced neuropathic pain in rats by targeting miR-133a-3p/SRPK1 axis

Fig. 4

SRPK1 was the downstream target of miR-133a-3p. a RT-qPCR analysis was conducted to detect the expression of target genes regulated by miR-216a-5p upregulation in rat microglial cells. b Luciferase reporter assay was conducted to explore the relationship between miR-133a-3p and SRPK1 in HEK-293T cells. c Expression of miR-133a-3p in rat microglial cells transfected with miR-133a-3p inhibitor or NC inhibitor was detected by RT-qPCR analysis. d RT-qPCR and western blot analyses were conducted to detect the mRNA or protein expression of SRPK1 in rat microglial cells by indicated transfections. e RT-qPCR analysis was conducted to assess the expression of SRPK1 in spinal cord tissues after CCI surgery for 0, 7, 14, 21 days. *p < 0.05

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Molecular Medicine

ISSN: 1528-3658