Fig. 2

Hdac3 diminishes the expression of the miR-17-92 cluster in BPD mice. a The expression of miR-17-92 cluster in lung tissues of different groups of mice assayed by RT-qPCR normalized to U6. b Western blot analysis of Hdac3 protein expression in cells after silencing of Hdac3 expression. c The expression of miR-17-92 cluster in cells following Hdac3 silencing assayed by RT-qPCR normalized to U6. d The enrichment of Hdac3 in the promoter region of miR-17-92 cluster in cells following different transfection. e Luciferase activity of miR-17-92 cluster promoter assayed by dual-luciferase reporter gene assay. *p < 0.05 compared to control mice. The above data were expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post-hoc test. n = 10 mice for each group