Fig. 6

Hdac3 upregulates Pgf expression through miR-17 inhibition to augment the abnormal alveolarization and pulmonary angiogenesis of BPD mice. a The expression of miR-17 in lung tissues of mice in different groups assayed by RT-qPCR. b The cell morphology in lung tissues of mice of different groups assayed by HE staining (× 400). c The MLI of alveolar in the lung tissues of mice in each group. d The changes in alveolar number in lung tissues of mice in each group. e Immunofluorescence assay of MVD in lung tissues of mice in each group (× 200). f The quantitation of expression of EZH1, p65, and Pgf in lung tissues of mice following different treatments assayed by immunohistochemistry. *p < 0.05 compared to untreated BPD mice. # p < 0.05 compared to mice subjected to hyperoxia + Hdac3^−/− + antagomir NC. The above data were expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post-hoc test. n = 10 mice for each group