Fig. 3

MiR-212-3p reduces VEGFA expression through SP1. a QRT-PCR detected expression level of miR-212-3p, Sp1 and VEGFA in MCF-7 and JIMT-1 after transfection of miR-212-3p mimics. b, c Western blot detected the protein level of Sp1 and VEGFA in MCF-7 and JIMT-1 after transfection of miR-212-3p mimics. d Binding site of miR-212-3p to Sp1. e Dual luciferase analysis of WT or MUT Sp1 in 293T cells transfected with miR-212-3p mimics. f QRT-PCR detected Sp1 and VEGFA mRNA levels in MCF-7 and JIMT-1 after Sp1 overexpression. g, h Western blot detected Sp1 and VEGFA protein levels in MCF-7 and JIMT-1 after Sp1 overexpression. i ChIP analysis of Sp1 and VEGFA promoter. Relative intensity of bands of each protein in western blot were normalized to loading control GAPDH. *P < 0.05, **P < 0.01, ***P < 0.001