Fig. 5

MiR-212-3p inhibited the proliferation and angiogenesis of BC cells by reducing the expression of VEGFA through SP1. a, b Proliferation ability of MCF-7 and JIMT-1 was detected by clone formation assay in cells transfected with miR-212-3p mimics and Sp1 (+). c, d Tube formation assay in MCF-7 and JIMT-1 cells transfected with miR-212-3p mimics and Sp1 (+). e, f Western blot detected VEGFA and MMP2 protein levels in MCF-7 and JIMT-1 cells transfected with miR-212-3p mimics and Sp1 (+). Relative intensity of bands of each protein were normalized to loading control GAPDH. *P < 0.05, **P < 0.01, ***P < 0.001