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Fig. 3 | Molecular Medicine

Fig. 3

From: MiR-128-3p inhibits vascular smooth muscle cell proliferation and migration by repressing FOXO4/MMP9 signaling pathway

Fig. 3

Effect of miR-128-3p on VSMCs. a CCK-8 assay was used to determine the viability of VSMCs treated with different concentrations of ox-LDL for 24 h. b CCK-8 assay was used to determine the viability of VSMCs treated with 100 mg/L ox-LDL for different stimulating times. c The transfection efficiency of miR-128-3p mimics and inhibitors was examined using qRT-PCR. The levels of inflammatory cytokines TNF-α (d), IL-1β (e) and IL-6 (f) in the supernatants of VSMCs were measured using ELISA. g, h VSMCs proliferation was measured using CCK-8 assay after the transfection. i VSMCs proliferation was measured using BrdU assay after the transfection. j VSMCs migration was examined using Transwell assay after the transfection. k VSMCs apoptosis was detected using flow cytometry after the transfection. *, **, *** represent p < 0.05, p < 0.01, p < 0.001, respectively

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